| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Sophia (12)
|
NIUN-D |
53.09 |
54.47 |
1.38 |
| 2 |
Noecker, Elle A (12)
|
NITWST |
57.41 |
57.24 |
-0.17 |
| 3 |
Er, Melissa (12)
|
NIUN-D |
1:07.30 |
1:00.45 |
-6.85 |
| 4 |
Krumpek, Ella E (12)
|
NITWST |
1:04.14 |
1:06.22 |
2.08 |
| 5 |
Kasubowski, Cassie A (11)
|
NIEAST |
1:10.67 |
1:09.73 |
-0.94 |
| 6 |
Christopher, Hailey R (12)
|
NIHAMB |
1:12.64 |
1:11.54 |
-1.10 |
| 7 |
Ziolkowski, Cosette M (12)
|
NITTAC |
1:16.89 |
1:14.95 |
-1.94 |
| 8 |
Delcamp, Ashlynn R (12)
|
NIHHSC |
1:17.32 |
1:16.62 |
-0.70 |
| 9 |
Sand, Makayla E (12)
|
NITWST |
1:17.15 |
1:19.20 |
2.05 |
| 10 |
Jusiak, Isabella N (12)
|
NIHAMB |
|
1:21.42 |
- |
| 11 |
Kerr, Bell L (11)
|
NIPVSC |
1:24.12 |
1:28.63 |
4.51 |